Animal laboratory clean environment control
- 2021-03-04
- 1308
- Esky Purify
Animal experimentation refers to the process of animal research through a long, experimental animal research value not only in promoting the development of biological sciences, but also a wide range of many areas of scientific experimental research closely linked to ensure that the modern scientific experimental research of an indispensable condition. Animal laboratories, also called laboratory animal rooms, are buildings suitable for keeping and breeding laboratory animals. Such buildings should have specific environmental requirements and experimental means to ensure the quality of animals and the accuracy and reliability of experimental research.
Laboratories can be categorized into four classes of laboratory animals according to microbiological control standards for laboratory animals, which are ordinary animals, clean animals, animals without special pathogens, and aseptic or amphibious animals. The corresponding facility classifications are open system, sub-barrier system, barrier system, and isolation system.
Animal Laboratory Classes |
Corresponding Class Requirements |
Class I General Animal (CV) |
Refers to general animals whose microorganisms are not subject to special control. It is required to exclude pathogens of zoonotic diseases and very few pathogens of virulent infectious diseases in laboratory animals. In order to prevent infectious diseases, in the experimental animal breeding and reproduction, to take certain measures, should ensure that the results of its use for testing has the reproducibility of the response (i.e., regardless of different operators, at different times, with the same strain of animals in accordance with the prescribed experimental procedures for the experiments, can be obtained almost the same results) |
Class II clean animals (CL) |
Required to exclude pathogens of zoonoses and major infectious diseases in animals |
Tier III Special Pathogen Free Animals (SPF) |
Requirements to Level II in addition to the exclusion of a number of specified pathogens. Its sterilization and disinfection methods, can use high-efficiency air filter sterilization, ultraviolet sterilization, triethylene glycol steam spray method and lithium chloride aqueous solution spray method |
Grade IV sterile animals (GF) or periphyton (GN) |
Sterile animals are required to be free of any microorganisms detectable by current methods. Periphyton requires the implantation of one or more known microorganisms on a sterile animal body. |
The cleanroom purification levels corresponding to the above systems are Class 300,000, Class 100,000, Class 10,000 and Class 100 (or localized Class 100 treatment in a Class 10,000 zone).
At present, SPF grade and GF grade animals are mainly used for scientific research, pharmacological animal experiments, etc. For a pharmaceutical enterprise only for the purpose of drug production quality testing of animal laboratories commonly used animals to ordinary grade, clean grade and SPF grade is mainly.
The importance of the construction of animal laboratory air system
1. Air purification system:because the animal room is a long time activities of experimental animals, indoor fumigation is not possible in the feeding conditions often with the sterilization of rinsing, generally only with a weak disinfectant scrub, in this case in order to avoid microbial infestation to ensure that the air purification is the first condition.
2. Deodorization:not only is the facility required for the external environment and staff, but also for the experimental animals themselves, because in addition to external microbial contamination indoor, animal excreta and microbial secondary conversion of ammonia, hydrogen sulfide, alcohol and other gases, but also an important pollutant, the stronger the odor of these gases, the lower the cleanliness of the animal room itself, generally increase the fresh air and activated carbon adsorption of two methods, the more common in recent years is to increase the chemical filtration, and the more common is to increase the chemical filtering. In recent years, it is more common to increase the chemical filtration device, deodorization and deodorization.
3. Animal rooms produce bad odors, odors, VOC and other harmful pollutant gases greatly affect the health of humans and animals:
-It can cause different degrees of harm to the organism and also interfere with animal experiments;
-When the concentration of ammonia increases, it can stimulate the cornea of the eyes, nasal mucosa and respiratory mucosa of animals and cause tears, coughing and other phenomena, and in serious cases, even produce acute pulmonary edema and cause death of animals.